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姓名:鲍锦库 性别:男
职称:教授 学院:生命科学学院
 
职务:
中国生物化学与分子生物学会糖复合物专业委员会委员、常务委员
中国生物化学与分子生物学会生物化学与分子生物学教学专业委员会委员、常务委员
中国生物工程学会糖工程专业委员会委员
国际凝集素委员会委员
联系方式:
办公电话:028-85415171
电子邮件:baojinku@scu.edu.cn
学位简历:
1987.09-1991.07:浙江大学生物系生物化学与微生物学专业,本科
1991.09 –1994.07:四川大生物系生物化学专业,理学硕士
2000.09 –2003.12:四川大学生命科学学院,理学博士
 
工作简历:
1994.07–1996.07:四川大学生命科学学院,助教
1994.09 –1995.09:
在中国农科院植保所植物病虫害生物学国家重点实验室和中国农科院畜牧研究所客座研究
1996.07–1998.07:
四川大学生命科学学院,讲师
1998.07–2005.07:
四川大学生命科学学院副教授
2005.07-2006.07:
四川大学生命科学学院教授博士生导师
2006.07 –至今:
四川大学生命科学学院教授,博士生导师。
 
个人科研工作简介
1)研究方向及主要学术成果:
立足我国丰富的天然药用生物资源宝库,发挥在天然药物研发的资源优势,围绕艾滋病等传染性疾病、临床常见恶性肿瘤等疾病及重大新药创制等国家战略需求,以具有特异糖结合能力的非免疫来源的凝集素为主线,集成现代生物技术手段,进行具有自主知识产权、抗病毒和抗肿瘤活性的凝集素等活性大分子的筛选、规模制备、基因克隆与蛋白序列、重组蛋白表达、生物活性及其基于肿瘤调控网络的分子机制、基于结构的分子设计和改造以及蛋白多肽数据库的构建和利用转基因植物大量获得活性蛋白等系统研究。发挥生命科学、医学和信息科学等学科特点及学科交叉优势,引入系统生物学整体性、信息化和网络调控的研究策略和理念,集成生物化学与分子生物学、细胞生物学、分子药理学、组学和系统生物学等技术方法,系统研究中草药蛋白多肽抗肿瘤分子机制及其潜在靶标,拓辟了“凝集素探针-肿瘤细胞程序性死亡相关蛋白-miRNA相互调控网络”研究新方向,为基于中药源活性蛋白多肽为先导或候选化合物的功能机制研究提供示范性的新思路并为源头创新药物研发提供通用模式。
 
2)科研项目:
国家自然科学基金项目:
1、寡糖链、氨基酸侧链基团与黄精凝集素生物学活性研究(批准号:39770178),1998 -2000,项目负责人
2、黄精凝集素结构与生物学活性关系研究(批准号:30000032),2001-2003,项目负责人
3、定点突变研究黄精凝集素II结构与功能关系(批准号:30270331),2003-2005,项目负责人
4、重组和突变抗艾滋病毒黄精凝集素II及其复合物晶体结构研究(批准号:30670469),2007-2009,项目负责人
5、黄精凝集素诱导肿瘤细胞自噬和凋亡的分子机制研究(批准号:30970643),2010-2012,项目负责人
6、凝集素诱导肿瘤细胞程序性死亡的分子机制(批准号:81173093),2012-2015, 项目负责人
7、十八届国际凝集素会议18th International Lectin Meeting, July, 1999, U.K),英国,项目负责人
8、第十九届国际凝集素会议(19th International Lectin Meeting, March, 2001, Brazil), 巴西,项目负责人
9、第二十届国际凝集素会议(20th International Lectin Meeting, May,2002,Denmark), 丹麦,项目负责人
10、第二十一届国际凝集素会议(21th International Lectin Meeting, May, 2004, Japan),日本,项目负责人
国家重大科技专项-转基因生物新品种培育:
“西南地区抗水稻胁迫因子基因克隆及功能验证”(2009ZX8009-072B)子课题负责人
国家高技术研究发展计划(863计划):
番茄营养品质改良的基因工程技术研究(2007AA10Z122),2007-2010,课题副组长
省部级课题:
1、中国农科院植物保护研究所植物病虫害生物学国家重点实验室开放课题:葱莲凝集素的基因克隆,2002,1-12,项目负责人
2、四川大学科技创新基金:我国传统中草药中具抗病毒活性的甘露糖结合蛋白的基因克隆与表达研究, 2004-2006,项目负责人
3、四川省杰出青年科技基金:转植物凝集素基因培养抗虫新品种(批准号:06ZQ026-035),2006-2008,项目负责人
4、四川省科技攻关项目:传统中草药中抗病毒抗肿瘤蛋白的分离、基因克隆表达及活性研究 (批准号:2006Z08-010),2006-2008,项目负责人
3)发表论文:
2012年:
Li WW, Li J, Bao JK*. Microautophagy: lesser-known self-eating. Cellular and Molecular Life Sciences. 2011. Doi: 10.1007/s00018-011-0865-5 (IF=7.047)
Wang Y, Wang H, Liu Y, Li C, Qi P, Bao J*. Antihyperglycemic Effect of Ginsenoside Rh2 by Inducing Islet β-cell Regeneration in Mice. Hormone and Metabolic Research. 2012. 44(1):33-40. (IF=2.414)
Xu HL, Li CY, He XM, Niu KQ, Peng H, Li WW, Zhou CC, Bao JK*. Molecular Modeling, Docking and Dynamic Simulation of GNA-related Lectins for Potential Prevention in Influenza Virus (H1N1). Journal of Molecular Modeling. 2012, 18(1):27-37. (IF 1.871)
Wang Y, Liu YD, Wang HL, Li CY, Qi P, Bao JK*. Agaricus bisporus lectins mediates islet β-cell proliferation through regulation of cell cycle proteins. Experimental Biology and Medicine. 2012, DOI: 10.1258 /ebm.2011.011251. (IF 2.954)
2011年
Liu JJ, Lin M, Yu JY, Liu B*, Bao JK*. Targeting apoptotic and autophagic pathways for cancer therapeutics. Cancer Letters. 2011. 300(2):105-114. (IF: 4.864) 总引次数 16 次,他引次数 12 次。
Wang SY, Yu QJ, Bao JK*, Liu B. Polygonatum cyrtonema lectin, a potential antineoplastic drug targeting programmed cell death pathways. Biochemical and Biophysical Research Communications. 2011. 406(4):497-500. (IF: 2.595) 总引次数 4 次,他引次数 2 次。
Li WW, Yu JY, Xu HL, Bao JK*. Concanavalin A: a potential anti-neoplastic agent targeting apoptosis, autophagy and anti-angiogenesis for cancer therapeutics. Biochemical and Biophysical Research Communications. 2011. 414(2):282-286. (IF: 2.595)
Li CY, Luo P, Liu JJ, Wang EQ, Li WW, Ding ZH, Mou L, Bao JK*. Recombinant expression of Polygonatum cyrtonema lectin with anti-viral, apoptosis-inducing activities and preliminary crystallization. Process Biochemistry. 2011. 46(2): 533-542. (IF: 2.648) 总引次数 2 次,他引次数 1 次。
Li CY, Wang EQ, Cheng Y, Bao JK*. Oridonin: An active diterpenoid targeting cell cycle arrest, apoptotic and autophagic pathways for cancer theraputics. The International Journal of Biochemistry & Cell Biology. 2011. 43(5):701-704. (IF: 4.956) 总引次数 4 次,他引次数 3 次。
Fu LL, Zhou CC, Yao S, Yu JY, Liu B, Bao JK*. Plant lectins: targeting programmed cell death pathways as antitumor agents. The International Journal of Biochemistry & Cell Biology. 2011. 43(10):1442-1449. (IF: 4.956) 总引次数 1 次,他引次数 1 次。
Yang Y, Xu HL, Zhang ZT, Liu JJ, Li WW, Ming H, Bao JK*. Characterization, molecular cloning, and in silico analysis of a novel mannose-binding lectin from Polygonatum odoratum (Mill.) with anti-HSV-II and apoptosis-inducing activities. Phytomedicine. 2011, 18(8-9):748-755. (IF 2.662)
He XM, Ji N, Xiang XC, Luo P, Bao JK. Purification, characterization, and molecular cloning of a novel antifungal lectin from the roots of Ophioglossum pedunculosum. Applied Biochemistry and Biotechnology. 2011. 165(7-8):1458-1472. (IF 1.879)
Yu QJ, Li ZY, Yao S, Ming M, Wang SY, Liu B, Bao JK. In silico analysis of molecular mechanisms of Galanthus nivalis agglutinin-related lectin-induced cancer cell death from carbohydrate-binding motif evolution hypothesis. Applied Biochemistry and Biotechnology. 2011. 165(3-4):1037-1046. (IF 1.879)
Zhang F, Li CY, Wang EQ, Li WW, Yu QJ, Wen X, Liu JJ, Bao JK*. The Global Component-Target Network in Panax Ginseng C. Letters in Drug Design & Discovery, 2011, 8, 181-188. (IF 0.668)
2010年
Liu B, Wu JM, Li J, Liu JJ, Li WW, Li CY, Xu HL, Bao JK*. Polygonatum cyrtonema lectin induces murine fibrosarcoma L929 cell apoptosis and autophagy via blocking Ras-Raf and PI3K-Akt signaling pathways. Biochimie. 2010. 92(12):1934-1938. (IF 4.864) 总引次数7次,他引次数1次。
Liu B, Bian HJ, Bao JK*. Plant lectins: Potential antineoplastic drugs from bench to clinic. Cancer Letters. 2010. 287(1):1-12. (IF 4.864) 总引用次数32,他引次数20次。
Yao Q, Wu CF, Luo P, Xiang XC, Liu JJ, Mou L, Bao JK*. A new chitin-binding lectin from rhizome of Setcreasea purpurea with antifungal, antiviral and apoptosis-inducing activities. Process Biochemistry. 2010. 45(9): 1477-1485. (IF: 2.648) 总引次数 4 次,他引次数 2 次。
Zhang ZT, Peng H, Li CY, Liu JJ, Zhou TT, Yan YF, Li Y, Bao JK*. Polygonatum cyrtonema lectin induces murine fibrosarcoma L929 cell apoptosis via a caspase-dependent pathway as compared to Ophiopogon japonicus lectin. Phytomedicine. 2010. 18(1):25-31. (IF 2.662) 总引次数 5 次,他引次数 1 次。
Li CY, Xu HL, Liu B, Bao JK*. Concanavalin A, from an old protein to novel candidate antineoplastic drug. Current Molecular Pharmacology. 2010. 3(3):123-128. EI文章 总引次数 6 次,他引次数 1 次。
Liu B, Cheng Y, Liu Q, Bao JK, Yang JM. Autophagic pathways as new targets for cancer drug development. Acta Pharmacologica Sinica. 2010. 31(9):1154-64. (IF 1.909) 总引次数 12 次,他引次数 4 次。
Ding J, Bao J, Zhu D, Zhang Y, Wang DC. Crystal structures of a novel anti-HIV mannose-binding lectin from Polygonatum cyrtonema Hua with unique ligand-binding property and super-structure. Journal of Structural Biology. 2010. 171(3):309-17. (IF 3.497) 总引次数 4 次,他引次数 1 次。
2009年
Liu B, Min MW, Bao JK*. Induction of apoptosis by Concanavalin A and its molecular mechanisms in cancer cells. Autophagy, 2009. 5(3): 432-433. (IF 6.643) 总引次数 19 次,他引次数 6 次。
Liu B, Cheng Y, Bian HJ, Bao JK*. Molecular mechanisms of Polygonatum cyrtonema lectin-induced apoptosis and autophagy in cancer cells. Autophagy. 2009. 5(2):253-255. (IF 6.643) 总引次数 26 次,他引次数 11 次。
Liu B, Cheng Y, Zhang B, Bian HJ, Bao JK*. Polygonatum cyrtonema lectin induces apoptosis and autophagy in human melanoma A375 cells through a mitochondria-mediated ROS-p38-p53 pathway. Cancer Letters. 2009. 275(1):54-60. (IF 4.864) 总引次数 45 次,他引次数 25 次。
Liu B, Li CY, Bian HJ, Min MW, Chen LF, Bao JK*. Antiproliferative activity and apoptosis-inducing mechanism of Concanavalin A on human melanoma A375 cells. Archives of Biochemistry and Biophysics. 2009. 482(1-2):1-6 (IF 3.022) 总引次数 24 次,他引次数 11 次。
Liu B, Zhang B, Min MW, Bian HJ, Chen LF, Liu Q, Bao JK*. Induction of apoptosis by Polygonatum odoratum lectin and its molecular mechanisms in murine fibrosarcoma L929 cells. Biochimica et Biophysica Acta (BBA) - General Subjects. 2009. 1790(8):840-844. ( IF 4.663) 总引次数 19 次,他引次数 6 次。
Peng H, Lv H, Wang Y, Liu YH, Li CY, Meng L, Chen F, Bao JK*. Clematis montana lectin, a novel mannose-binding lectin from traditional Chinese medicine with antiviral and apoptosis-inducing activities. Peptides. 2009. 30(10):1805-1815. (IF 2.654) 总引次数 12 次,他引次数 9 次。
Lv H, Kong Y, Yao Q, Zhang B, Leng FW, Bian HJ, Balzarini J, Van Damme E, Bao JK*. Nebrodeolysin, a novel hemolytic protein from mushroom Pleurotus nebrodensis with apoptosis-inducing and anti-HIV-1 activities. Phytomedicine. 2009. 16(2-3):198-205. (IF 2.662) 总引用次数7次,他引次数4次。
Chen J, Liu B, Ji N, Zhou J, Bian HJ, Li CY, Chen F, Bao JK*. A novel sialic acid-specific lectin from Phaseolus coccineus seeds with potent antineoplastic and antifungal activities. Phytomedicine. 2009. 16(4):352-360. (IF 2.662) 总引用次数23次,他引次数15次。
Liu B, Peng H, Yao Q, Li J, Van Damme E, Balzarini J, Bao JK*. Bioinformatics analyses of the mannose-binding lectins from Polygonatum cyrtonema, Ophiopogon japonicus and Liparis noversa with antiproliferative and apoptosis-inducing activities. Phytomedicine. 2009. 16(6-7):601-608. (IF 2.662) 总引用次数15次,他引次数3次。
Ye SH, Chen S, Zhang F, Wang W, Tian Q, Liu JZ, Chen F, Bao JK*. Transgenic tobacco expressing Zephyranthes grandiflora agglutinin confers enhanced resistance to aphids. Applied Biochemistry and Biotechnology. 2009. 158(3):615-30. (IF 1.879) 总引用次数10次,他引次数9次。
Li CY, Wang Y, Peng H, Bian HJ, Min MW, Chen LF, Liu Q, Bao JK*. In silico Analysis of the Potential Infection Mechanisms of Magnaporthe grisea from Horizontal Gene Transfer Hypothesis. Genomics, Proteomics & Bioinformatics. 2009. 7(3):77-86.
Li CY, Meng L, Liu B, Bao JK*. Galanthus nivalis agglutinin (GNA)-related lectins: Traditional proteins, Burgeoning drugs? Current Chemical Biology. 2009. 3:324-333.
EI 总引次数 9 次,他引次数 1 次。
Zhao Q, Wu CF, Wang WG, Yuan S, Bao JK, Chen F. In vitro plantlet regeneration system from rhizomes and mannose-binding lectin analysis of Polygonatum cyrtonema Hua. Plant Cell, Tissue and Organ Culture. 2009. 99(3): 269-275. (IF 1.243)
2008年
Liu B, Xu XC, Cheng Y, Huang J, Liu YH, Liu Z, Min MW, Bian HJ, Chen J, Bao JK*. Apoptosis-inducing effect and structural basis of Polygonatum cyrtonema lectin and chemical modification properties on its mannose-binding sites. BMB Reports. 2008. 41(5):369-375. (IF 2.167) 总引用次数24次,他引次数1次。
Liu C, Zhao X, Xu XC, Li LR, Liu YH, Zhong SD, Bao JK*. Hemagglutinating activity and conformation of a lactose-binding lectin from mushroom Agrocybe cylindracea. International Journal of Biological Macromolecules. 2008. 42(2):138-144. (IF 2.502) 总引用次数6次,他引次数4次。
Liu Z, Liu B, Zhang ZT, Zhou TT, Bian HJ, Min MW, Liu YH, Chen J, Bao JK*. A mannose-binding lectin from Sophora flavescens induces apoptosis in HeLa cells. Phytomedicine. 2008. 15(10):867-875. (IF 2.662) 总引用次数31次,他引次数16次。
Tian Q, Wang W, Miao C, Peng H, Liu B, Leng FW, Dai L, Chen F, Bao JK*. Purification, characterization and molecular cloning of a novel mannose-binding lectin from rhizomes of Ophiopogon japonicus with antiviral and antifungal activities. Plant Science. 2008. 175(6): 877-884. (IF 2.481) 总引次数 13 次,他引次数 4 次。
Xu XC, Zhong SD, Kai F, Li LR, Liu C, Liu B, Bao JK*. Preparation and characterization of a novel recombinant human parathyroid hormone (1-34) analog (Gly1-Gln26-rhPTH(1-34)) with enhanced biological activity. Protein & Peptide Letters. 2008. 15(8):854-860. (IF 1.849) 总引次数 0 次,他引次数 0 次。
Ding JJ, Bao JK, Zhu DY, Zhang Y, Wang DC. Crystallization and Preliminary X-Ray Diffraction Analysis of a Novel Mannose-Binding Lectin with Antiretroviral Properties from Polygonatum cyrtonema Hua. Protein and Peptide Letters. 2008. 15(4):411-414. (IF 1.849) 总引次数 4 次,他引次数 1 次。
2007年
Luo YT, Xu XC, Liu JW, Li J, Sun YS, Liu Z, Liu JZ, Van Damme E, Balzarini J, Bao JK*. A novel mannose-binding tuber lectin from Typhonium divaricatum (L.) Decne (family Araceae) with antiviral activity against HSV-II and anti-proliferative effect on human cancer cell lines. Journal of Biochemistry and Molecular Biology. 2007. 40(3):358-367. (IF 2.021) 总引用次数23次,他引次数14次。
Liu JW, Xu XC, Liu JZ, Balzarini J, Luo YT, Kong Y, Li J, Chen F, Van Damme E, Bao JK*. A novel tetrameric lectin from Lycoris aurea with four mannose-binding sites permonomer. Acta Biochimica Polonica. 2007. 54(1):159-166. (IF 1.234) 总引用次数6次,他引次数3次
Xu XC, Wu CF, Liu C, Luo YT, Li J, Zhao XP, Damme EV, Bao JK*. Purification and Characterization of a Mannose-Binding Lectin from the Rhizomes of Aspidistra elatior Blume with Antiproliferative Activity. Acta Biochimica et Biophysica Sinica (Shanghai). 2007. 39(7): 507-519 (IF 1.547) 总引用次数14次,他引12次
学术专著
Huai-long Xu, Lei-lei Fu, Yu Yang, Bo Liu, Xu Zhao, Wen-Wen Li, Jin-Ku Bao. Autophagy pathways and drug targets in cancer. In: Autophagy: Principles, Regulation and Roles in Disease. Accepted for Publication. August, 2011. Nova Science Publisher. Hauppauge NY, USA。
鲍锦库,刘博,《自噬:生物学与疾病》第二十八章:针对自噬的药物开发和治疗的展望。2011年3月 科学出版社。
其它论著
An J, Liu JZ, Wu CF, Li J, Dai L, Van Damme E, Balzarini J, De Clercq E, Chen F, Bao JK*. Anti-HIV I/II activity and molecular cloning of a novel mannose/sialic acid-binding lectin from rhizome of Polygonatum cyrtonema Hua. Acta Biochimica et Biophysica Sinica (Shanghai). 2006. 38(2):70-8. (IF 1.547) 总引用次数26次,他引次数8次。
Wu CF, Li J, An J, Chang LQ, Chen F, Bao JK*. Purification, Biological Activities, and Molecular Cloning of a Novel Mannose-Binding Lectin from Bulbs of Zephyranthes candida Herb (Amaryllidaceae). Journal of Integrative Plant Biology. 2006. 48(2):223-231. (IF 1.603)
Gao S, An J, Wu CF, Gu Y, Chen F, Yu Y, Wu QQ, Bao JK*. Effect of amino acid residue and oligosaccharide chain chemical modifications on spectral and hemagglutinating activity of Millettia dielsiana Harms. ex Diels. Lectin. Acta Biochimica et Biophysica Sinica (Shanghai). 2005. 37(1): 47-54. (IF 1.547)
Gong M, An J, Lü HZ, Wu CF, Li YJ, Cheng JQ, Bao JK*. Effects of denaturation and amino acid modification on fluorescence spectrum and hemagglutinating activity of Hericium erinaceum Lectin. Acta Biochimica et Biophysica Sinica (Shanghai). 2004. 36(5):343-350 (IF 1.547)
Wu CF, An J, He XJ, Deng J, Hong ZX, Liu Chao, Lu HZ, Li YJ, Wang CJ, Chen F, Bao JK*. Molecular Cloning of a Novel Mannose-binding Lectin Gene from Bulbs of Amaryllis vittata (Amaryllidaceae). Acta Botanica Sinica. 2004. 46(11):1301-1306.
Jiang FB, Du J, Yu XQ, Bao JK, Zeng XC. Metallomicellar catalysis: accelerated hydrolysis of BNPP by copper(II), zinc(II), and nickel(II) complexes of long alkanol-imidazole in CTAB micellar solution. Journal of Colloid and Interface Science. 2004. 273(2):497-504. (IF 3.066)
Cai YF, Zhang H, Zeng Y, Mo JC, Bao JK, Miao C, Bai J, Yan F, Chen F. An optimized gossypol high-performance liquid chromatography assay and its application in evaluation of different gland genotypes of cotton. Journal of Biosciences. 2004. 29(1):67-71. (IF 1.888)
Biological Activity Analysis of Polygonatum cyrtonema Hua. Lectin by Site-Directed Mutagenesis, Trends in Glycoscience and Glycotechnology, 2004,16:pp.s1–s32(IF 0.863)
In Vitro Plantlet Regeneration from Rhizome Lumps and Mannose-Binding Lectin Analysis of Polygonatum cyrtonema Hua.(Family Liliaceae) ,Trends in Glycoscience and Glycotechnology(TIGG), 2004,16: pp.S 33–S 64(IF 0.863)
Miao C, Gou XP, Lan LQ, Bao JK, Xu Y, Wang SH, Chen F. Molecular cloning and expression of RSSSG58 gene in rice sperm cells. Acta Botanica Sinica. 2003. 45(2):234-241.
Xiang QX, Zhou H, Wang XY, Yu XQ, Bao JK, Xie RG. Interaction between Bifunctional Kioxo Macrocyclic Polyamine Co(II) Complex and DNA in Metallomicellar System. Chinese Chemical Letters. 2002. 13(3):223-226. (IF 0.775)
Bao JK, Zeng ZK. Purification and biological activity of a lectin from Polygonatum cyrtonema Hua. Eur J Cell Biol 1997, 74(Suppl.46): 8. (IF 3.630)
Bao JK, Zhou H, Zeng ZK. Conformation and activity of Millettia dielsianna Harms lectin, Eur J Cell Biol,1997,74(Suppl.46):8(IF 3.630)
 
国家发明专利:
1、一种大量获得黄精凝集素II的方法。专利号:ZL 2004 1 0021659.1
2、黄精凝集素Ⅱ蛋白在制备治疗或预防乙型肝炎的药中的应用。专利号:ZL 2004 1 0022354.2
3、黄精凝集素II蛋白在制备治疗或预防单纯疱疹病毒所致疾病的药中的应用。专利号:ZL 2004 1 0022353.8
4、黄精凝集素II蛋白在制备治疗或预防艾滋病的药中的应用。专利号:ZL 2006 1 0005577.7
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四川大学生命科学学院学科介绍
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